2013年8月6日星期二

lactic fermentation Rhizopus


Rhizopus oryzae is a genus of L-lactic acid bacteria fermentation representation, shaped fermentation, at home and abroad For Rhizopus oryzae breeding work made a lot of coverage. Yu et al for direct fermentation of agricultural Rhizopus oryzae
L-lactic acid production were studied calcium carbonate as neutralizing agent, per kg of the original crude starch Expected to generate more than 350g L-lactic acid. And applied for a U.S. patent. Jiangsu Province microbiological research
The study by Cao Chang et al bred out of an L-lactic acid producing strain Rhizopus JSM1 an R73, and The properties of the strain and acid conditions for the study with 500L Lactic acid been expanded Experiment. When the glucose concentration was 10% acid 7Og / L; 13%, the production of acid 101g / L to
On corn meal can be used as the Yin medium, when the concentration of corn flour 12% acid production 70g / L or more; 20% of the acid-105g / L, where L-lactic acid purity of 88%, Up to 99%. Shanxi Institute of Microbiology Jiang Pearl and others from 56 kinds of Rhizopus Strains were screened out 10 kinds of high yield strains of lactic acid, which Rhizopus R-47 L-lactic acid producing the highest
Acid stable conditions in shake flask culture, the initial glucose concentration was 15%, 3 ~ C, 48h, L a lactic acid up to 118.4g / L, for sugar conversion rate of 78.9%. Yang Hong, Fuzhou University Starch an acidic medium using the enrichment culture, combined with potassium buffered lactic acid  a potassium bromide tablet detection flower Screening methods produce lactic acid from the soil 81g / L of a two strains of Rhizopus R, with its strain by UV mutagenesis, plates obtained from succinic acid R a 2-91, using glucose as carbon source Under acid 103g / L, for sugar conversion rate of 68.9%. Chinese Academy of Sciences Institute of Plasma Gusau Bin glucose as carbon source for the PW352 strain characteristics were studied, and on this basis, using Ion implantation method Rhizopus oryzae PW352 was modified to obtain high yield L-lactic acid bacteria
RE3303, acid production capacity of 131 ~ 136g / L, up to 140g / L, the conversion of glucose.86% to 90%, acid production increased 75% compared PW352. Tianjin 3 - Industry Institute of Microbiology Young Sub-culture and others with Rhizopus oryzae TL a 527 - 9 strains of corn starch deep fermentation production of L a milk Acids. 3m in fermenter, when the concentration of corn starch 130g / L, the average amount of acid 94. 1g / L, the conversion rate of 79.55% sugar, wherein the purity of L-lactic acid content of up to 98% Above, the study has a high industrial application value.

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